Figure 1From: A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibitionDistinction between norovirus amplicons and internal control amplicon allowed by the melt curve analysis. The internal control has a melting temperature around 81.5°C, human norovirus amplicons, around 85.5°C and bovine norovirus amplicons has a melting temperature around 88.5°C. Such differences in temperature are clearly visible on the curve.Back to article page