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Table 1 Listing of the compounds screened for a modulating effect on viral persistence in DRAW cells.

From: Modulation of viral replication in macrophages persistently infected with the DA strain of Theiler's murine encephalomyelitis virus

Compound Established effect, virus (strain) Reference Effect on TMEV yield from DRAW Cmaxd  
2-AP a, TMEV (GDVII) [21] -c 200 μg/ml  
L-NAME ↑, CVB (CVB3) [22] - 250 μg/ml  
L-NMMA ↑, CVB (CVB3) [23] - 250 μg/ml  
2-FMC b, HRV (HRV2) [24] - 0.1 μg/ml  
hydantoin ↓, PV (Mahoney) [25] - 20 μg/ml  
levamisole ↓, EMCV [26] - 200 μg/ml  
pirodavir ↓, HRV (HRV9) [27] - 10 μg/ml  
Compound Established effect, virus (strain) Reference Log10 maximal increase or decrease
of TMEV yield from DRAWe
EC50 e EDf
hemin ↑, PV (Mahoney) [28] ↑; 0.99 ± 0.23 13 μg/ml 65 μg/ml
anti-TMEV mAb ↓, TMEV (DA, GDVII) [29] ↓; 1.12 ± 0.04 1:250 dilution 1:10 dilution
enviroxime ↓, HRV (HRV31) [30] ↓; 0.99 ± 0.13 0.1 μg/ml 0.316 μg/ml
IFN-α ↓, TMEV (DA) [21] ↓; 4.89 ± 0.23 10 ng/ml 250 ng/ml
IFN-γ ↓, TMEV (DA) [21] ↓; 5.89 ± 0.49 0.2 ng/ml 25 ng/ml
  1. a ↑: increase of infectivity; b ↓: decrease of infectivity; c -: no influence on infectivity; d Cmax: highest, non-cytotoxic concentration; e EC50: effective concentration50 = concentration of the compound inducing a twofold increase or decrease of infectious virus yield from DRAW cells, as determined by titration in L929 cells; f ED: effective dose = concentration that maximally affected infectious virus yield from DRAW cells; CVB: coxsackievirus B; EMCV: encephalomyocarditis virus; HRV: human rhinovirus; PV: poliovirus; TMEV: Theiler's murine encephalomyelitis virus. Data are the mean result of duplicate samples from two independent experiments ± standard deviation