Figure 3
From: Expression of frog virus 3 genes is impaired in mammalian cell lines
Codon optimized 75L expresses in BGMK cells. A FV3-75L construct tagged with a C-terminal myc tag under the control of a CMV promoter (A) or a codon optimized construct (B) was transfected into BGMK cells. Twenty-four hours post-transfection cells were fixed and indirect immunofluorescence was performed to detect 75L (anti-myc:green) and differential interference contrast (DIC) was used to visualize the cell. Images were captured on a laser scanning confocal microscope. (C) The optimized sequence is shown above the original 75L sequence, with altered nucleotides shown in red. The corresponding amino acids are shown on the bottom row and are the same for both the original and optimized sequence.