Figure 1

FV3 infected BGMK and HeLa cells do not express the gene 75L. FHM, HeLa, and BGMK cells were infected with FV3 at an MOI of 1. At 0, 3, 8, 16, and 32 hours post-infection, cells were fixed and a FV3 infection was detected using anti-FV3 antibodies (blue: A, C, E) and 75L was detected using anti-FV3-75L antibodies (green: B, D, F). No images of FHM cells at 32 hours were taken as the cells had succumbed to infection. Cells were visualized using DIC and indirect immunofluorescence images were captured on a laser scanning confocal microscope.