Figure 5

Inhibition of post entry HIV-1 replication. (A) SupT1 cells were infected for 1.5 hours in the absence of any treatment, with HIV-1 chimera NL4-3 Env^-Luc⁺/VSV-G pseudotype, washed 3 times, and then treated with increasing concentrations of SP4-2, for 24 h. Intracellular luciferase gene marker expression was quantified from cell lysates that were normalized to the same number of viable cells by the MTT assay, and percent inhibition of HIV-1 replication was calculated from a control cell culture of infected but untreated cells, and plotted on the y-axis. (B) Standard cell free fluorescent RT assay was performed in the presence of 2 units recombinant HIV-1 RT/reaction with the indicated concentrations of SP4-2. Percent inhibition was calculated comparative to assay performed in absence of treatment, 100% RT activity. Data are mean ± SD of three separate experiments.