Figure 2

Inhibition of X4 and R5-tropic HIV-1. GHOST X4/R5 and GFP expressing cells were plate at 1 × 10⁵/well in 12-well plates and incubated at 37°C in CO₂ atmosphere with increasing concentrations of SP4-2, as indicated, then infected with either X4-tropic NL4-3 (panel A, a-d) or with R5-tropic 81A (panel B, e-h), at 0.3 moi, in replicates (n = 4). 48 h after infection cells were quantified by FACS, and % infected cells is shown on each panel. Uninfected and untreated control (mock) is superimposed over each graph in dotted line. Representative of 4 experiments.