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Table 1 Identification of neuraminidase subtypes of influenza A clinical nasopharyngeal aspirates.

From: A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus

Sample
No. *
HA
subtypea
NA
subtype
Subtype Amplification by
our RT-PCR
sequence
1 H3 NVI1 H3 - -
2 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
3 H3 NVI1, N/A2 H3 + N2
5 H3 N2 H3N2 Wisconsin/67/2005 like strain + -
6 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
8 H3 N2 H3N2 + N2
9 H3 NVI1, N/A2 H3 - -
10 H3 N2 H3N2 + N2
12e H1 N1 H1N1 + N2
14 H3 N2 H3N2 Wisconsin/67/2005 like strain - -
15 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
17 H3 NVI1, N/A2 H3 + NSA3
19 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
21 H3 NVI1, N/A2 H3 + N2
26 H3 N2 H3N2 Wisconsin/67/2005 like strain - -
27 H3 NVI1 H3 - -
30 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
31 H3 NVI1 H3 + NSA3
32 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
33 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
34 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
36 H3 NVI1 H3 - -
37 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
38 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
42 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
45 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
47 H3 NVI1, N/A2 H3 - -
50 H3 NVI1, N/A2 H3 - -
51 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
54 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
55 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
56 H3 NVI1, N/A2 H3 - -
57c NVI1 NVI1, N/A2 Not available - -
62d H5 N/A2 H5 + N2
63d H5 N/A2 N/A2 - -
64d Not H5 N/A2 N/A2 - -
65 H3 N2 H3N2 Wisconsin/67/2005 like strain + N2
67f - N/A2 Not available + NSA3
  1. *Blinded specimens provided by Pathology Queensland. Only typed A influenza samples were listed. Type B influenza samples, adenovirus, and RSV samples were not included as they were all negative by our assay.
  2. a HA subtyping was performed by Queensland Forensic and Scientific Services using real-time PCR based on HA specific primers, as well as by hemaglutination and inhibition test to the viruses that were able to be cultured.
  3. c Sample 57 was not confirmed by a second laboratory for Influenza A infection nor was there virus isolated.
  4. d Samples 62, 63 and 64 are not clinical samples but are positive control RNA sample from WHO reference lab.
  5. e Sample was assayed and subtyped in duplicate to corroborate the NA subtype by sequence.
  6. f Sample 67 had pink contaminants floating in the RNA that could have affected PCR result.
  7. NVI1 = No virus isolated, therefore NA subtyping was unfeasible.
  8. N/A2 = Sample was not assayed with the corresponding test.
  9. NSA3 = RT-PCR was positive but we were not able to obtain subtype by direct sequencing.