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Figure 3 | Virology Journal

Figure 3

From: A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus

Figure 3

One-step RT-PCR amplification of NA gene from all 9 NA subtypes using animal samples from allantoic fluids. A fragment of approximately 253 bp was amplified using primers containing M13 sequence. Example of some subtypes (refer to Table 2 for strain names) assayed: M, 100 bp DNA Ladder (Promega); 1) H9N2, 2) H16N3, 3) H8N4, 4) H14N5, 5) H13N6, 6) H10N7, 7) H11N9, 8) H5N1, 9) negative control (water instead of template), 10) H6N5, 11) H13N6, 12) H14N6, 13) H7N7, 14) H3N8, 15) H11N9, 16) negative control (water instead of template).

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