Table 9 Env, gag and pol primer details used in the nested PCR's
From: Recombination in feline immunodeficiency virus from feral and companion domestic cats
Gene region and PCR round | Primer Name | Location (bp) | PCR product length (bp) |
|---|---|---|---|
env – round one | VE1S | 7134–7154* | 1230 |
|  | VE1R | 8364–8345 |  |
   - round two | VE2S | 7326–7345 | 858 |
|  | VE2R | 8184–8165 |  |
gag – round one | LTR1 | 122–141** | 1287 |
|  | Gag548 | 1409–1389 |  |
   - round two | LTR2 | 285–309 | 1127 |
|  | Gag548 | 1409–1389 |  |
pol – round one | 1258F | 2430–2451** | 577 |
|  | 1260R | 3007–2988 |  |
   - round two | 1259F | 2466–2488 | 524 |
|  | 1261R | 2990–2968 |  |