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Table 9 Env, gag and pol primer details used in the nested PCR's

From: Recombination in feline immunodeficiency virus from feral and companion domestic cats

Gene region and PCR round Primer Name Location (bp) PCR product length (bp)
env – round one VE1S 7134–7154* 1230
  VE1R 8364–8345  
   - round two VE2S 7326–7345 858
  VE2R 8184–8165  
gag – round one LTR1 122–141** 1287
  Gag548 1409–1389  
   - round two LTR2 285–309 1127
  Gag548 1409–1389  
pol – round one 1258F 2430–2451** 577
  1260R 3007–2988  
   - round two 1259F 2466–2488 524
  1261R 2990–2968  
  1. * using TM2 as reference sequence
  2. ** using Petaluma as reference sequence