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Table 3 Oligonucleotide primers used for amplification of LASV genes expressed in E. coli

From: Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance

LASV Gene Amplified

LASV Primer

Oligonucleotide Primer Sequence

GP1

5' GP1 bac

TTTCAGAATTCGGATCC ACCAGTCTTTATAAAGGGGTTTAT

GP1

3' GP1 bac

GGTACCAAGCTT TCA GTCA TAGCAATCTTCTACTAATATAAATATCTCT

GP2

5' GP2 bac

TTTCAGAATTCGGATCC GGCACATTCACATGGACACTG

GP2

3' GP2 bac

GGTACCAAGCTT TCA GCTA TGTCTTCCCCTGCCTCTCCAT

NP

5' NP bac

TTTCAGAATTC AGTGCCTCAAAGGAAATAAAATCCTTTTTGTGGACACAATCTTTGAGGAGGGAATTATCTGGTTAC

NP

3' NP bac

GGTACCAAGCTT TCA GTTA CAGAACGACTCTAGGTGTCGATGT

  1. Note. REN sites are underlined, and stop codons (TCA, CTA, TTA) are in bold print.
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Virology Journal

ISSN: 1743-422X