Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance

Table 2 Cross reactivity of Old and New World arenavirus-specific MHAFs against recombinant LASV GP1, GP2, and NP proteins by Western blot and ELISA

Western blot (WB) and ELISA were performed with 100 ng of purified E. coli-expressed NP (NP bac), GP1 (GP1 bac), and GP2 (GP2 bac). WB abbreviations: (-) negative, no visible band detected; (+/-) faint band detected; (+) bright band detected; (++) very bright band detected. ELISA abbreviations (all signals are respective to corrected background): (-) negative; (+/-) < 2X; (+) > 2X < 3X; (++) > 3X < 4X; (+++) > 4X < 5X.

ISSN: 1743-422X