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Table 2 Cross reactivity of Old and New World arenavirus-specific MHAFs against recombinant LASV GP1, GP2, and NP proteins by Western blot and ELISA

From: Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance

   NP bac GP1 bac GP2 bac
MHAF Distribution WB ELISA WB ELISA WB ELISA
NMS - - - - - - -
LCMV Old World +/- - - - - -
Ippy Old World ++ +/- - - ++ +++
Mobala Old World ++ ++ + + ++ ++
Mopeia Old World ++ ++ - +/- + ++
Latino New World - - - - - -
Tamiami New World +/- - - +/- +/- +/-
Pirital New World + +/- - - - -
Pichinde New World + - ++ - + +/-
Oliveros New World +/- - - - +/- -
  1. Western blot (WB) and ELISA were performed with 100 ng of purified E. coli-expressed NP (NP bac), GP1 (GP1 bac), and GP2 (GP2 bac). WB abbreviations: (-) negative, no visible band detected; (+/-) faint band detected; (+) bright band detected; (++) very bright band detected. ELISA abbreviations (all signals are respective to corrected background): (-) negative; (+/-) < 2X; (+) > 2X < 3X; (++) > 3X < 4X; (+++) > 4X < 5X.
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