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Table 1 N-terminal sequencing of LASV proteins expressed in E. Coli

From: Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance

LASV Protein

Protein Form

N-terminal sequence

NP

Full length – 55 kDa

ISEF SASKEI

NP

Truncated – 46 kDa

ISEF SASKEI

GP2

Full length – 20 kDa

ISEFGS GTFT

GP2

Truncated – 13 kDa

ISEFGS GTFT

GP1

Full Length – 22 kDa

ISEFGS TSLYK

  1. The N-terminus of each recombinant LASV protein cleaved with Factor Xa contains four extraneous amino acids for NP and six for GP1 and GP2 prior to the start of the arenaviral protein sequence. The extraneous amino acids are in italics and the arenaviral protein sequences are in bold.
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Virology Journal

ISSN: 1743-422X