Figure 4

Western blot analysis of p53 and MAGI-3 levels in the presence of HPV type 18 E6-GFP, type 33 E6-GFP and type 33 variants E6-GFP as indicated. Upper panel: Western blot analysis of p53 and MAGI-3 (anti-V5 antibodies). Lower panel: Western blot analysis of E6-GFP. Note that HPV-33 E6 variant 2 was the most effective at mediating the degradation of both p53 and MAGI-3 and HPV-33 E6 variants 7 and 8 were the least effective.