In vitro response of Vero and SK-N-SH cells to Se deficiency. Vero and SK-N-SH cells were grown in Se-deficient (Se-) and Se-adequate (Se+) conditions as described in the materials and methods. Equal number of cells were seeded in 96-well plates and growth curve was measured by cell counting of control, Se-, and Se+, Vero (A) and SK-N-SH (B) cells for 5 days post-seeding. (C) Cell viability of Vero and SK-N-SH cells at day 7 of the induction of Se deficiency was assessed by cell proliferation assay and percentage cell viability of Se- and Se+ cells was calculated by comparing to control cells. Data are expressed as mean ± SD from two separate experiments performed in triplicate.