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Figure 3 | Virology Journal

Figure 3

From: Down-regulation of cell surface CXCR4 by HIV-1

Figure 3

Immunofluorescence microscopy analysis of CXCR4 expression in permeabilized HIV-1 and mock infected RH9 cells. Four days after HIV-1 infection, cells were fixed, permeabilized with saponin and labeled with a mouse monoclonal antibody to CXCR4 (12G5) and a secondary, FITC-conjugated anti-mouse antibodies for observation with a fluorescence microscopy. Panel A: Immunofluorescence staining control with isotype-matched monoclonal antibody. Panel C: CXCR4 immunofluorescence staining of H9 cells. Panels E and G, CXCR4 immunofluorescence staining of HIV-1 infected H9 cells. Panels B, D, F and H show phase contrast images of the same fields of cells shown in left panels.

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