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Figure 1 | Virology Journal

Figure 1

From: Down-regulation of cell surface CXCR4 by HIV-1

Figure 1

Flow cytometry analysis demonstrating reduced CXCR4 expression in HIV-1 infected RH9 cells. Panel A: RH9 T-lymphoblastoid cells infected with HIV-1LA1. On days 1, 4, and 7 postinfection cells were fixed with 4% paraformaldehyde, stained with mouse MAb 12G5 anti-CXCR4 (10 μg/ml) or isotype-matched control antibody followed by fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse immunoglobulin G, and analyzed by flow cytometry. Median fluorescence intensity was calculated as an indicator of the level of cell surface CXCR4 expression. Data are presented as single-color histograms with FITC fluorescence (CD3 expression) along the horizontal axis and relative cell number along the vertical axis. RH9 cells (control cells), heavy solid line: H9 cells infected with HIV, dotted line; H9 with an isotype-matched control antibody, thin solid line. Panel B: Analysis of surface CD3 expression in HIV-1 and mock infected RH9 cells by FACS analyzed on day 7 post-infection.

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