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Figure 4 | Virology Journal

Figure 4

From: The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay

Figure 4

Inhibition of HIV-1 activity by crude pregnancy plug mucus and purified pregnancy plug mucins in vitro assay. Crude pregnancy plug mucus and purified pregnancy plug mucins (0.9 mg each) were incubated with subtype D HIV-1 for 60 min and filtered through 0.45 μm pore size cellulose acetate filter. As controls HIV-1 treated with media and heat inactivated HIV-1 were used. The unfiltered samples were then incubated with CEM SS cells at a concentration of 0.5 × 106cells ml-1 for 30 min, 1 h and 3 h. After PBS wash cells were cultured and viral replication was measured by a qualitative p24 antigen assay. Letters a, b, c and d indicate the anti-HIV-1 activity of each sample in a serial tenfold dilution of 10-1, 10-2, 10-3 and 10-4 respectively. P. plug represents pregnancy plug.

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