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Figure 1 | Virology Journal

Figure 1

From: The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay

Figure 1

Caesium chloride density gradient purification of the pregnancy plug mucins. Samples in 4 M GuHCl were adjusted to a density of 1.39 to 1.40 g ml-1 with solid caesium chloride. Density gradient centrifugation was performed in a Beckman L45 ultra-centrifuge for 48 h at a 105 000 g at 4°C. Mucin positive fractions (◆) at a density (▲) between 1.37–1.42 and still associated with some protein (■) (a) were pooled and prepared for the second step centrifugation (b). Finally fractions (fraction number 3, 4 and 5) were pooled, dialysed against three changes of distilled water and freeze-dried.

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Virology Journal

ISSN: 1743-422X