Figure 7

Functional amino acids of P1 involved in silencing suppression. (A) ClustalW alignment of P1 proteins with the putative Zn-finger (in grey) and detection of potential amino acids involved in silencing suppression that were point mutated (in box). (B) Northern analysis of gus-siRNA and mRNA at 2 days post delivery (dpd) with sTz3, and mutated sTz3 (F88Y, N19I, C64S and C95S) in the del or dis parts of the leaf (see Figure 5A). 35S is an empty cassette (negative control) and EtBr staining of rRNA served as a loading control. Relative accumulation to the loading control are shown, RNA band intensities were quantified using Image Quant software (Molecular Dynamics).