Figure 6
From: Genetic diversity and silencing suppression effects of Rice yellow mottle virus and the P1 protein
Movement of P1 protein and silencing suppression. (A) Schematic representation of the experimental design to distinguish delivery (del) and distal (dis) points after bombardment. (B) Northern analysis of gus-siRNA and mRNA at 2 days post delivery (dpd) with sTz3, Mg1 and CI63 P1s in the del or dis parts of the leaf. 35S is an empty cassette (negative control) and EtBr staining of rRNA served as a loading control. (C) Western blot analysis of P1 protein. Relative accumulation to the loading control are shown, RNA band intensities were quantified using Image Quant software (Molecular Dynamics).