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Figure 4 | Virology Journal

Figure 4

From: Genetic diversity and silencing suppression effects of Rice yellow mottle virus and the P1 protein

Figure 4

Heterogeneous silencing suppression caused by isolates from a homogeneous phylogenetic group (i.e. S2 isolates), at 40 dpi. (A) Northern blot analysis of gus-siRNA. Controls group together: positive controls for gus-siRNA detection corresponding to RNA from L10 either NI (non-inoculated) or BI (buffer-inoculated) and RNA from non transgenic Tai, or transgenic L4 plants as negative controls. EtBr staining of rRNA served as a loading control. (B) GUS activity is measured by fluorimetry and is expressed as a percentage of the maximal activity measured in CI110 inoculated plants. Analyses were carried out with soluble protein extracted from L10 leaves infected by different isolates. Data represent average values of two independent measurements with standard deviations indicated. (C) Western blot analysis of P1 protein.

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