Figure 5

Plaque size comparison between rpOka and rpOkaΔ58 in MRC-5 cells. Plaque sizes after infection with cell-free rpOka and rpOkaΔ58 are indicated graphically (upper) and in photographs (lower). MRC-5 cells were infected with each cell-free virus and cultured for 10 days. The cells were fixed and stained with 1% crystal violet/70% EtOH and the sizes of 38 plaques (rpOka) and 41 plaques (rpOkaΔ58) were calculated and analyzed using ImageJ software (NIH, USA). Error bars in the graph indicate the standard deviation (SD).