Figure 4

Confirming the expression of ORF58 by RT-PCR. rpOka or rpOkaΔ58-infected cells were harvested at 24 hrs p.i., and the RNAs were extracted. The cDNAs were synthesized from each RNA using Superscript III (Invitrogen), and PCRs were performed using the cDNAs as templates. PCRs were also performed with RT(-) to avoid amplification of contaminating genomic DNA. The size of each product is indicated by an arrow at the right of each panel. The molecular size (bp) is shown on the left of each panel. N.C.: negative control.