Figure 1
From: Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture
Construction of recombinant virus rpOkaΔ58. (A) The VZV genome consists of two unique regions (UL and US) and of inverted repeat sequences (IRL, IRS, and TRSs) flanking the US region. An enlarged section shows the analyzed portion of the genome, containing open reading frame (ORF) 56, 57, 58 and 59. ORFs are drawn as pointed rectangles. (B) A fragment consisting of the 3'UTR of ORF58, the kanamycin-resistance gene (kmr), and the 5'UTR of ORF58 was amplified by PCR and used for mutagenesis of an infectious full-length pOka genome in E. coli and named KmrΔ58. (C) The entire ORF58 gene was replaced by the kanamycin-resistance gene in E. coli. TRL, terminal repeat long; UL, unique long; IRL, internal repeat long; IRS, internal repeat short; Us, unique short; TRS, terminal repeat long.