Figure 3

Detection of NS1 protein in supernatant media from cells treated with lysosomotropic agents. C6/36 HT cells infected with dengue 2 virus at MOI of 10 were either treated for 1 h, before infection (-1 hpi) or 10 min post-infection (10 mpi) with 50 mM NH₄Cl (open bars). As a control, non drug treated cells, either mock infected or infected, were run in parallel (grey bars). At 24 hpi the supernatant media were collected and tested for the presence of NS1 protein with Platelia™ Dengue NS1 Ag kit. Results are expressed as the percentage of the OD (A₄₅₀) obtained in non-drug treated, infected cells. Results are mean values ± SD of three independent experiments. The decrease in NS1 production in drug treated cells was statistically significant compared to the control condition (* p < 0.05). Titers for each condition obtained by plaque assay and expressed as PFU/ml are shown on the top of the bars.