Figure 3

p53 represses E2-induced transcription. The graphs show the levels of luciferase activity found in (A) HeLa and (B) SAOS-2 cell extracts 24 hrs after transient co-transfection with an E2-responsive reporter plasmid and plasmids expressing E2 or E2m1 and p53. Promoter activity was normalized with respect to transfection efficiency as in Figure 1 and is shown as fold activation over the reporter alone. The results are the average and standard deviation of four experiments.