Figure 1

Mutagenesis of the HPV 16 E2 protein. (A) A molecular model of the dimeric DNA binding domain of the HPV 16 E2 protein [49] produced using RasMol 2.7.3.1 [50] and showing the amino acids mutated in this study: D338 (red), E340 (green), W341 (blue) and D344 (yellow). (B) The table shows the amino acids changes made in mutants E2m1 to E2m8. E2m1 was formerly referred to as E2p53m. (C) The graph shows the levels of luciferase activity found in HeLa cell extracts 24 hrs after transient co-transfection with an E2-responsive reporter plasmid and plasmids expressing the E2 proteins described above. Promoter activity was normalized with respect to transfection efficiency using a co-transfected plasmid expressing Renilla luciferase and is shown as fold activation over the reporter alone. The results are the average and standard deviation of four experiments.