Dissecting the role of putative CD81 binding regions of E2 in mediating HCV entry: Putative CD81 binding region 1 is not involved in CD81 binding

Table 1

HCVpp	Infect.	Infectivity (%)		Cellular Express.	HCVpp E1E2 Detection		E1E2 Assoc.	CD81-GST Binding	Conform.	Group
		Huh7	Hep3B	E2	E1	E2
WT	+++	100	100	+++	++	+++	+	+++	+++	wt
Y474A	++	77	36	+++	+++	++++	+	+++	NA	wt&III
D481A	+++	64	71	+++	+	++++	++	+++	NA	wt
R483A	-	2	1	+++	+	++++	++	++++	good	III
Y485A	-	2	1	+++	+	++++	+	+++	good	III
W487A	-	1	0	+++	-	++++	+	+++	+++	II
H488A	-	2	1	+++	+++	++++	+	+++	good	III
Y489A	-	4	2	+++	+++	+++	++	++++	good	III
R492A	-	5	2	+++	+	++++	+	+++	NA	III
Y527A	-	5	1	+	++++	+	+	-	+++	I
W529A	-	1	1	++	++++	++++	+++	-	++	I
D533A	-	12	5	++	++++	++++	+	+++	+++	III
D535A	-	2	0	++	+++	+++	++	-	-	IV
W549A	-	2	0	+	-	++++	++++	+++	+++	II
F550A	-	19	2	++	+	+++	++++	++	good	III
Y613A	-	3	1	+	+	+++	++	-	+++	I
R614A	-	3	0	+	++	++++	++++	-	-	IV
L615A	+	44	10	+	+	++++	+++	++	NA	III
W616A	-	2	0	+	+	+++	+	-	-	IV
H617A	-	2	0	+	++	+	+++	-	+	I
Y618A	-	2	1	+	+	++++	+++	+/-	+	I

+ and - denote the properties of wt and mutants of HCV E2
"NA" indicates mutations not screened in this study and "good" indicates mutants screened in previous study [45]

ISSN: 1743-422X