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Figure 4 | Virology Journal

Figure 4

From: Dissecting the role of putative CD81 binding regions of E2 in mediating HCV entry: Putative CD81 binding region 1 is not involved in CD81 binding

Figure 4

Binding of mutant HCV E1E2 glycoproteins to soluble CD81. (A) 293T cells transfected with HCV E1E2 wt or mutant expression vectors were lysed 24 h post-transfection. Cleared cell lysate was incubated with soluble CD81-GST fusion protein. Binding to CD81 was determined by Western Blot analysis of E2 and the GST tag. As a negative control, GST protein without soluble CD81 was incubated with HCV wt. Image is a composite. (B) 293T cells transfected with HCV E1E2 wt or specific mutant expression vectors were lysed 24 h post-transfection. Cleared cell lysate was incubated with AR3A (C1) conformational antibody to assess conformation of mutations. Immunoprecipitated proteins were detected by subsequent Western Blot analysis of E2. Image is a composite.

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Virology Journal

ISSN: 1743-422X