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Figure 3 | Virology Journal

Figure 3

From: Dissecting the role of putative CD81 binding regions of E2 in mediating HCV entry: Putative CD81 binding region 1 is not involved in CD81 binding

Figure 3

Expression and incorporation of HCV E1E2 glycoproteins in producer cell lysate and HCVpp. (A) 293T HCVpp producer cells were lysed and analyzed by Western Blot analysis using anti (α)-E2 and (α)-actin antibodies. Image is a composite. (B) Incorporation of HCV glycoproteins into HCVpp was determined by pelleting the virus through a 20% sucrose cushion followed by Western Blot analysis. HCV glycoproteins were identified with (α)-E2 and (α)-E1 antibodies. Detection of the HIV p24 capsid protein with an anti-HIV p24 antibody was performed as a loading control. Image is a composite.

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Virology Journal

ISSN: 1743-422X