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Table 1 Subtype-specific antibody responses are evident in sera from A/PR/8/34(H1N1) and A/Wuhan/95(H3N2)-infected animals.

From: Antibody contributes to heterosubtypic protection against influenza A-induced tachypnea in cotton rats

  Antibody titer as measured by a
  HAI NI Neutralization Neut + C1q
Serum source H1N1 H3N2 H1N1 H3N2 H1N1 H3N2 H1N1 H3N2
Naïve serum 10 10 0 0 100 100 100 100
H1N1-immune 640 10 80 0 1600 100 3200 100
H3N2-immune 10 160 0 320 100 200 100 800
  1. aStandard hemagglutination inhibition (HAI), neuraminidase inhibition (NI) and neutralization (neut) assays in the absence as well as presence of complement factor C1q were performed as described in Materials and Methods. Viruses used for these assays were A/PR/8/34 (H1N1) and A/Wuhan/359/95 (H3N2) that had been used to infect the cotton rats that were the source of this serum pool. Animals were boosted several times by rechallenging them with the same virus before serum was collected. The lowest dilution of serum used in the HAI assay was 1/10 and therefore no inhibition of agglutination is recorded as a titer of < 10. The lowest dilution of serum used in the neutralization assay was 1/100 and therefore no neutralization is recorded as a titer of < 100.