Figure 4
From: HDAC inhibitors stimulate viral transcription by multiple mechanisms
Association of p300 with transcribing SV40 minichromosomes after HDACi treatment. Unfixed SV40 minichromosomes treated with 250 μM NaBu or 120 nM TSA were isolated from cells infected with 776 wild-type virus for 48 hours, immunoprecipitated with RNAPII, and then subjected to an ISFIP/ReChIP analysis with antibody against p300. The samples were amplified by simplex PCR with primer sets to the late region. The position of the amplification product from the wild-type 776 DNA is indicated. Lane 1, ISFIP input fraction; lane 2, ChIP with 7.5 μl of hyperacetylated histone H4 antibody (ISFIP); lane 3, ChIP with 10 μl of p300 antibody (ISFIP); lane 4, ReChIP input fraction; lane 5, ChIP with 7.5 μl of hyperacetylated histone H4 antibody.