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Table 1 VV Δ F2L-gfp exhibits virulence characteristics that are similar to the parent virus.

From: Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug (N)-methanocarbathymidine

Virus (PFU/mouse)a Mortality MDDb
  Number Percent  
VV-WR, UAB    
1.6 × 104 10/10 100 6.8 ± 0.8
1.6 × 103 3/10 30 8.3 ± 0.6
1.6 × 102 0/10 0 ---
16 0/10 0 ---
1.6 0/10 0 ---
VV-WR, Turner    
Stock 1.6 × 108 10/10 100 3.2 ± 0.4
1.6 × 107 10/10 100 3.9 ± 0.3
1.6 × 106 10/10 100 5.1 ± 0.3
1.6 × 105 10/10 100 6.2 ± 0.4
1.6 × 104 10/10 100 7.9 ± 1.0
VV Δ F2L-gfp    
Stock 6 × 107 10/10 100 4.2 ± 0.4
6 × 106 10/10 100 4.7 ± 0.5
6 × 105 10/10 100 6.1 ± 0.6
6 × 104 10/10 100 7.1 ± 0.3
6 × 103 5/10 50 8.4 ± 0.5
  1. a. Anesthetized mice were inoculated intranasally with 40 μl of virus (20 μl/nostril).
  2. b. Mean day of death (MDD) is shown with the standard deviation.