Figure 2

Concentration dependence of mAb-N5D3-binding capacity of monomeric NPs immobilized on membrane. The solutions containing the radiolabeled polymeric (p-NP) and monomeric (m-NP) NPs were repeatedly spotted in a volume of 10 μl onto nitrocellulose. As a result, the indicated increasing amount of NP were loaded into the dots. The dots were then subjected to immunostaining (A, upper and middle row), autoradiography (A, lower row) and densitometric analysis (B). Densitometric analysis of the spots (B) shown in Fig. 2A: white columns – immunodetection of p-NP corresponding to the upper row ; dotted columns – immunodetection of m-NP corresponding to the middle row; grey columns – autoradiographs of m-NP corresponding to the lower row.