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Table 1 Transcript levels of viral genes on ISAV segment 8 in extended haemagglutination assays1

From: Infectious salmon anaemia virus replication and induction of alpha interferon in Atlantic salmon erythrocytes

Sampling points in days NBISA01 haemagglutination RPC/NB-04-085-1 haemagglutination
  One-step RT-PCR with F5/R5 primer pair RT with oligodT primer & PCR with F5/R5 primer pair One-step RT-PCR with F5/R5 primer pair
0 26.57 ± 0.14 22.22 ± 0.05 20.39 ± 0.21
1 24.43 ± 0.15 20.63 ± 0.05 21.23 ± 0.15
2 22.25 ± 0.21* 19.40 ± 0.02 20.46 ± 0.26
3 21.51 ± 0.33* 18.31 ± 0.02* 20.76 ± 0.36
4 21.04 ± 0.40* 18.67 ± 0.27* 20.27 ± 0.22
5 20.48 ± 0.29* 19.29 ± 0.12* 20.55 ± 0.29
  1. 1Ct values are average ± SD of triplicate observation.
  2. *denotes more than 3.3 Ct difference in the starting sample concentrations from that of the 0 hour.