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Table 1 Transcript levels of viral genes on ISAV segment 8 in extended haemagglutination assays1

From: Infectious salmon anaemia virus replication and induction of alpha interferon in Atlantic salmon erythrocytes

Sampling points in days

NBISA01 haemagglutination

RPC/NB-04-085-1 haemagglutination

 

One-step RT-PCR with F5/R5 primer pair

RT with oligodT primer & PCR with F5/R5 primer pair

One-step RT-PCR with F5/R5 primer pair

0

26.57 ± 0.14

22.22 ± 0.05

20.39 ± 0.21

1

24.43 ± 0.15

20.63 ± 0.05

21.23 ± 0.15

2

22.25 ± 0.21*

19.40 ± 0.02

20.46 ± 0.26

3

21.51 ± 0.33*

18.31 ± 0.02*

20.76 ± 0.36

4

21.04 ± 0.40*

18.67 ± 0.27*

20.27 ± 0.22

5

20.48 ± 0.29*

19.29 ± 0.12*

20.55 ± 0.29

  1. 1Ct values are average ± SD of triplicate observation.
  2. *denotes more than 3.3 Ct difference in the starting sample concentrations from that of the 0 hour.
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Virology Journal

ISSN: 1743-422X