Skip to main content
Figure 1 | Virology Journal

Figure 1

From: Mapping of immunogenic and protein-interacting regions at the surface of the seven-bladed β-propeller domain of the HIV-1 cellular interactor EED

Figure 1

Crystallogenesis of histidine-tagged isoform 3 of EED. (A), Platelet-like crystals of EED3-H6 protein of 441 residues, obtained in suspended drop in 0.1 M MES buffer pH 6.0, 40 % MPD. (B), Solubilization of the crystals and analysis by SDS-PAGE and Coomassie blue staining. Lane 1 : solution of purified EED3-H6 (10 mg/mL) used for crystallogenesis (protein load : 50 μg). Lane 2 : protein content of solubilized single crystal. MW : markers of protein molecular mass, indicated in kilodaltons (kDa) on the right side of the panel.

Back to article page