A) Hydropathic analysis of the HPV16 E5α protein using a window of 9 amino acids and the Kyte&Doolittle index. HR: hydrophobic region. B) Schematic representation of the mutants employed in our experiments. Sequences were cloned into the Eco Rl-Bam Hl restriction sites of the vector pFlag-CMV4. In all cases the start methionine amino acid was deleted. All recombinants were confirmed by sequencing.