Table 1 Primers used in TULV isolate-specific RT-PCR assays.
Primer name (isolate, segment, forw/rev) | Sequence 5'-3' | Position (nt) | Amplicon size (bp) |
|---|---|---|---|
LVSF783 (Lodz, S, forw) | GAAAAAGCAAGGTGGTCCCAAC | 783–804 | 266 |
LVSR1026 (Lodz, S, rev) | GGATTGAGAAGAAGGCTCCTAAT | 1026–1048 | |
LodzG2F426 (Lodz, M, for) | CAAATTGAGGTCAGTCGGG | 426–444 | 528 |
LodzG2R953 (Lodz, M, rev) | AATGATAAATCCCTATTGACG | 933–953 | |
LodzG2F554 (Lodz, M, nested PCR, forw) | CCGTTAAAGTTTGCATGATAGGG | 554–576 | 261 |
LodzG2R814 (Lodz, M, nested PCR, rev) | GTTGATAGCCAGAAACTGTATTG | 792–814 | |
TulSF895 (Moravia, S, forw) | GATTGATGACTTGATTGATCTTGC | 895–918 | 255 |
MVSR1149 (Moravia, S, rev) | GCGTCTCAGATATGACTGATAG | 1128–1149 | |
MorG2F83 (Moravia, M, forw) | CTGATTTAGAATTGGATTTTTCCC | 83–106 | 735 |
MorG2R817 (Moravia, M, rev) | TTCTCTGATATCCAGATACAGTG | 795–817 | |
MorG2F444 (Moravia, M, nested PCR, forw) | CAAAGTTTATAAAATCCTGTCCC | 444–466 | 136 |
MorG2R579 (Moravia, M, nested PCR, rev) | TGTTCCAATCATACAGACCTTC | 558–579 |