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Figure 3 | Virology Journal

Figure 3

From: Tula hantavirus isolate with the full-length ORF for nonstructural protein NSs survives for more consequent passages in interferon-competent cells than the isolate having truncated NSs ORF

Figure 3

Detection of TULV in MRC5 cells infected with the supernatant from double-infected Vero E6 cells. MRC5 cells were infected with the passage 1 supernatant from Vero E6 cells infected with the mixture of TULV/Lodz and TULV/Moravia. Supernatant was used to infect fresh cells, and from them RNA was isolated. RT-PCR was done with the isolate-specific S- and M-primers. From top: results of RT-PCR assays with the primers specific for: TULV/Lodz S segment, TULV/Lodz M segment, TULV/Moravia S segment, and TULV/Moravia M segment.

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Virology Journal

ISSN: 1743-422X