La Crosse virus infectivity, pathogenesis, and immunogenicity in mice and monkeys

Table 2 LACV is immunogenic in rhesus monkeys and can be detected in the lymph nodes.

				Homologous neutralizing antibody titer on indicated day (reciprocal PRNT₆₀)^c
Virus and inoculation route	Monkey #	Temp. (°F)^a	Lymph node virus titers^b	-7	0	2	4	6	8	10	12	14	21	28	GMT^d (Day 28)
Human/1978/clone (SQ)	DB00		2.3 (day 12)	<5	<5	<5	<5	<5	13	22	166	620	1465	2924	2186
	DB1Z			<5	<5	<5	<5	<5	24	137	126	332	679	1412
	CL74			<5	<5	<5	<5	<5	<5	31	199	546	1370	1374
	DBCV			<5	<5	<5	<5	<5	6	43	246	234	1684	4022
Human/1978/clone (IM)	DB9J			<5	<5	<5	<5	<5	<5	66	343	1011	603	1776	1906
	DB5C		1.4 (day 12)	<5	<5	<5	<5	<5	<5	74	282	2382	7451	2714
	DB7Y	103.2 (day 8)		<5	<5	<5	<5	<5	<5	15	37	230	4041	1628
	DB3W	102.8 (day 6)		<5	<5	<5	<5	<5	<5	40	1643	460	2633	1688
Mosquito/1978/clone (SQ)	DB70			<5	<5	<5	<5	37	25	90	623	672	2325	3008	560
	DB8N		0.7 (day 12)	<5	<5	<5	<5	12	29	72	194	161	211	663
	CK74			<5	<5	<5	<5	<5	14	38	250	434	1023	399
	CL2G			<5	<5	<5	<5	7	7	8	19	14	120	124
Mosquito/1978/clone (IM)	DBCZ			<5	<5	<5	<5	12	38	834	1325	3050	3695	4493	1691
	CL6E	103.1 (day 6)	0.7 (day 6)	5	6	6	<5	16	39	180	1960	2809	3875	960
	DB0H			13	13	18	13	15	28	130	516	1566	1134	687
	DA9F	102.9 (day -7)	0.7 (day 8)	<5	<5	<5	<5	<5	11	94	1833	3189	3656	2762

^a Rectal temperature collected at time of blood draw. Temperatures outside the normal range (98–102.7 F) indicated by day in parenthesis, normal values left blank.
^b Lymph nodes were collected on days 4, 6, 8, and 12 for virus titer and IHC staining. Viral titers are expressed as log₁₀ PFU/half lymph node (day post inoculation). Viral antigen was not observed using IHC staining. Blank spaces indicate that virus was not detected in the lymph nodes. Lower limit of detection was .7 log₁₀ PFU/tissue.
^c Limit of detection for neutralizing antibodies is 1:5 dilution.
^d Geometric mean neutralizing antibody titer.

ISSN: 1743-422X