Figure 5

Viral antigen is detected in the CNS of LACV infected mice. (A) LACV antigen-positive cells in the mitral cell layer (arrow) and granule cell layer (arrowhead) of the main olfactory bulb. Abbreviations: AL-airway lumen, OE-olfactory epithelium, LP-lamina propria, TB-turbinate bone, ONL-olfactory nerve layer, GL-glomerular layer, EPL- external plexiform layer, M-mitral cell layer, GR-granule cell layer. Day 6, X100. (B) Low magnification of a coronal section of mouse brain showing abundant La Crosse viral antigen. Abbreviations: C-cerebral cortex, CAM-cornu ammonis of hippocampus, DG-dentate gyrus, HPA-posterior hypothalamic area, MP-premamillary nucleus, PAG-periaqueductal gray, PT-pretectum, R-reticular nucleus of thalamus, ZI- zona inserta. Day 6, X12.5. (C) Cervical spinal cord cross section showing abundant La Crosse viral antigen in grey matter. Abbreviations: GC-gray commissure, DH-dorsal horn, LH-lateral horn, VH-ventral horn. Day 6, X100. (D) Viral antigen in a punctate pattern in neurons (arrows) in the locus coeruleus. Day 5, X400. (E) Medulla oblongata with abundant LACV antigen in many neurons (arrow heads) with associated perivascular lymphocyte cuffing (indicated with arrows). Day 4, X200. (F) La Crosse viral antigen in the cytoplasm of medullary neurons (arrows). Day 6, X1000. All images immunohistochemistry, hematoxylin counterstain.