Histopathologic changes on day 6 in the CNS of LACV infected mice. (A) Perivascular cuffs and gliosis in the thalamus. H&E X400. (B) Neurons of the cervical spinal cord with degenerative changes of pale cytoplasm and vacuoles (arrows). H&E X1000. (C) Thalamus with apoptotic cells (arrows) and degenerative neurons (with swollen vacuolated nuclei). H&E X1000. (D) CD3+ lymphocytes in the meninges and blood vessels in the thalamus. Immunohistochemistry, hematoxylin counterstain, X200. (E) Macrophages (MAC-2 +) in perivascular cuffs and areas of gliosis in the hippocampus. Immunohistochemistry, hematoxylin counterstain X200.(F) TUNEL positive (brown) apoptotic bodies in the thalamus. Immunohistochemistry, hematoxylin counterstain X1000.