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Table 1 Kinetic parameters for the NS5A:Fyn SH3 domain interaction.

From: Hepatitis C virus NS5A protein binds the SH3 domain of the Fyn tyrosine kinase with high affinity: mutagenic analysis of residues within the SH3 domain that contribute to the interaction

Protein

ka (M-1s-1)

kd (s-1)

KD (M-1)

NS5A(Δ32)

4.93 × 103

3.08 × 10-3

6.29 × 10-7

(± SD) n = 3

(± 0.66 × 103)

(± 0.16 × 10-3)

(0.59 × 10-7)

NS5A(Δ250)

9.05 × 103

4.94 × 10-3

5.56 × 10-7

(± SD) n = 3

(± 1.62 × 103)

(± 0.11 × 10-3)

(0.59 × 10-7)