Wild type measles virus attenuation independent of type I IFN

Table 2 Pathogenicity of G954 MV strains in vivo

Mice genotype (no. of mice)	Viral strain	Mortality rate (time/days)	MV replication (10 days pi) (*)		anti-N response (15 days pi)(†)
			brain	lung
C57/Bl6 (8)	G954 PBL	0 %	-	-	-
	G954 V13	0 %	-	-	-
CD150 tg (6–8)	G954 PBL	100% (9–15 d)	+++	+	++
	G954 V13	0%	-	-	+/++
	UV inactivated G954 V13	0%	nd	nd	-
CD150/IFNARKO (8–10)	G954 PBL	100% (9–11 d)	nd	nd	nd
	G954 V13	0%	nd	nd	+/++

(*)determined by RT PCR on N mRNA: +++ > 10× housekeeping gene expression; + > 0,1× housekeeping gene expression ; – beyond limit of detection ; nd not determined
(†) determined by ELISA on N specific sera antibodies: ++ between 1 and 10 arbitrary units; + between 0,1 and 1 arbitrary units; – beyond 0,1 arbitrary units

ISSN: 1743-422X