Table 2 Primers for cloning VL and VH of mAb 54.6 into P. pastoris expression vector pPICZαA
From: Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands
VKF-54.6 | 5'- CAC GAA TTC GAC ATC TTG CTG ACT CAG TCT CCA GCC -3' |
VKLR-54.6 | 5'- CTA CGG TAC CCT TAC CTC CAG ATC CCT TAC CTC CTT TGA TTT CCA ACT TGG TGC C -3' |
VHLF-54.6 | 5'- CAC GGG TAC CGG AGG TAA GGG ATC TGG AGG TAA GGG ATC TGA AGT GAA ATT GGT GGA GTC -3' |
VHR-54.6 | 5'- GGC ACT CTA GAG AGG AGA CAG TGA GAG TGG TGC C -3' |