Figure 5
From: Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands
scFv54.6 blocks the binding of rNV VLPs to CHO-FTBKE cells. rNV VLPs were incubated with CHO-FTBKE cells in the presence or absence of scFv54.6 and sorted by flow cytometry (A) VLP binding detected by incubation with scFv54.6 followed by anti-c-myc mAb and PE-conjugated goat anti-mouse antibody. (B) VLP binding detected by incubation with anti rNV MAb 72.1 PE-conjugated goat anti-mouse and C) scFv54.6 were pre-incubated with VLPs for one hour prior to addition to the cells. Bound VLPs were detected by MAb 72.1.