Figure 4
From: Ephrin-B2 expression critically influences Nipah virus infection independent of its cytoplasmic tail
Influence of EB2 overexpression on NiV and control MV Edm infection. (A) Vero cells transfected with the indicated amounts of pCAGGS-EB2 were infected with NiV at a MOI of 1 at 15 h after transfection. At 24 h p.i., cells were fixed and an immunostaining was performed using a NiV-specific guinea pig antiserum and rhodamine-conjugated secondary antibodies. Nuclei were visualized by DAPI staining. (B) Nuclei per syncytium were quantified as described in the legend to Fig. 1D. (C) To determine the amount of initially NiV-infected cells, the total number of NiV-positive syncytia on each coverslip was counted. (D) Virus titers in the supernatant were determined by the TCID50 method at 24 h p.i.. (E) Vero cells transfected with different quantities of pCAGGS-EB2 were infected with MVEdm at a MOI of 1. Virus titers in the supernatant were determined by plaque assay at 42 h p.i..