Figure 3From: Ephrin-B2 expression critically influences Nipah virus infection independent of its cytoplasmic tailSurface expression of NiV and MV glycoproteins in the presence of increasing amounts of EB2. Vero cells were cotransfected with constant amounts of NiV F- or G-encoding plasmids and the indicated amounts of pCAGGS-EB2. At 24 h p.t., cells were surface biotinylated and lysed. (A) Immunoprecipitation of NiV G was carried out using a polyclonal NiV antiserum. After separation on a 12% SDS gel under reducing conditions and blotting to nitrocellulose, surface-biotinylated G proteins were detected by IRDye 800-conjugated streptavidin using a LiCor-Odyssey imager. (B) NiV F was immunoprecipitated with an F-specific antiserum, separated by SDS-PAGE under non-reducing conditions and further processed as described above. (C) Vero cells were cotransfected with constant amounts of plasmids encoding the MV F and H proteins and the different amounts of pCAGGS-EB2. Immunoprecipitation of the MV glycoproteins was carried out using F- and H-specific antibodies. After separation by SDS-PAGE under reducing conditions and blotting, proteins were detected as described above.Back to article page