Figure 2

NiV and MV glycoprotein-mediated cell-to-cell fusion in Vero cells overexpressing different amounts of EB2 or CD46. (A) Vero cells were cotransfected with plasmids encoding the NiV F and G protein and increasing amounts of pCAGGS-EB2. Cell-to-cell fusion was visualized by Giemsa staining at 24 h p.t.. (B) Quantification of syncytium formation was performed as described in the legend to Fig. 1D. (C) Cells transfected with constant amounts of the MV glycoproteins and the indicated amounts of EB2 were Giemsa stained at 15 h p.t.. (D) Cells were transfected with constant amounts of plasmids encoding NiV F and G proteins and different amounts of plasmids encoding the CD46 gene. At 24 h p.t., syncytium formation was visualized by Giemsa staining.