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Figure 2 | Virology Journal

Figure 2

From: Uncoupling GP1 and GP2 expression in the Lassa virus glycoprotein complex: implications for GP1 ectodomain shedding

Figure 2

Intracellular expression of LASV GP1TM, sGP1, and wild type GPC from mammalian vectors driven by CMV MIE intron-A containing or intronless constructs. Ten micrograms of total protein from HEK-293T/17 cell extracts (C) transfected with each DNA construct were resolved on SDS-PAGE gels, blotted onto nitrocellulose membranes and probed with a mix of LASV GP1-specific mAbs and an HRP-conjugated goat α-mouse IgG antibody. Protein expression from CMV intron-A containing GP1-TM (lane 1) and sGP1 (lane 2) constructs were compared to intronless counterparts (lane 3, GP1-TM; lane 4, sGP1). An empty plasmid control, pcDNA3.1(+):intA, is shown in lane 5. Wild type GPC expressed from an intron-A containing construct (lane 7) was compared to an intronless counterpart (lane 8). Expression of GPC was detected with a mix of LASV GP1 and GP2-specific mAbs and an HRP-conjugated goat α-mouse IgG antibody. Protein molecular weight sizes in kDa are indicated to the right of the panel.

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